Difference between revisions of "PCR polymerase chain reaction"
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Latest revision as of 19:36, 4 August 2008
This is a machine to replicate a chunk of DNA
There are primers that are short strips of nucleotides constructed to bind to known sequence sections of a DNA
DNA is heated to 95C and that splits the helix into two strands. Into the soup of floating strands, primers are introduced.
Temp is reduced to 50C, allowing binding
A 3-5 primer "1" binds to the 5 end of one strand and 5-3 primer"2" binds to the 3 end of the other strand.
Nucleotides start binding to the 5 end of the primer and to the DNA, essentially recreating the strands.
After a bit they have filled in from both ends, a strand (and complement) has been replicated.
Now the new strands are separated by heating to 95C.
More primer binds and the cycle repeats.