https://wiki2.sitebuilt.net/index.php?action=history&feed=atom&title=PCR_polymerase_chain_reaction 2026-05-06T17:31:42Z Revision history for this page on the wiki MediaWiki 1.37.0 https://wiki2.sitebuilt.net/index.php?title=PCR_polymerase_chain_reaction&diff=1894&oldid=prev 2008-08-04T23:36:08Z

<p></p> <p><b>New page</b></p><div>This is a machine to replicate a chunk of DNA<br /> <br /> There are primers that are short strips of nucleotides constructed to bind to known sequence sections of a DNA<br /> <br /> DNA is heated to 95C and that splits the helix into two strands. Into the soup of floating strands, primers are introduced. <br /> <br /> Temp is reduced to 50C, allowing binding<br /> <br /> A 3-5 primer &quot;1&quot; binds to the 5 end of one strand and 5-3 primer&quot;2&quot; binds to the 3 end of the other strand. <br /> <br /> Nucleotides start binding to the 5 end of the primer and to the DNA, essentially recreating the strands. <br /> <br /> After a bit they have filled in from both ends, a strand (and complement) has been replicated. <br /> <br /> Now the new strands are separated by heating to 95C.<br /> <br /> More primer binds and the cycle repeats.</div>

Tim